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Biomedicum Genomics Oy
support@bmgen.fi
Haartmaninkatu 8
FI-00290 Helsinki
Finland
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Instructions for Sample Submission - Recombinant Virus Services
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Viral products viral particles, biosafety and virus titer analyses are
ordered by filling out the Virus Particle Order Form and virus construct information sheet (.xls). BMGen will
then track down and inform you about available pLKO.1 shRNA (or shRNAmir if requested) lentiviral
vectors corresponding to requested genes and make a quote. We also accept
customer’s own lenti- and retroviral shRNA or cDNA vectors for viral
particle production.
General viral particle order instructions
When submitting the virus order form (pdf), please notify of desired
insert type (shRNA, shRNAmir or cDNA), virus type (lenti- or retrovirus)
and production volume of viruses (Mini, Midi or Maxi). Fill out your
client information (name, institution / company, research group /
department, address, phone, email) into the separate excel sheet. For
extended service including cell line production, please
contact us.
All virus particle sets ordered through Biomedicum Genomics will be
quality controlled by p24 virus titer analysis.
Viral particles can be ordered in three different classes:
- VSVg lentivirus particles
- amphotropic retrovirus
particles
- ecotropic retrovirus particles
and in four
different volumes:
- Mini scale (1,5 ml)
- Midi scale (6 ml)
- Maxi scale
(20 ml)
- Concentrated (180 µl and 380 µl)
For further
information see Products and
Services.
Customers who request viral particle production from their own transfer
vector should contact BMGen for details. For virus production we need the
following amount of vector DNA per one construct:
- Mini scale 10 µg
- Midi scale 18 µg
- Maxi scale 30
µg
- Concentrated 30 µg or 60 µg
When submitting the Virus Particle Order Form please have the following
information available:
- Client contact information (name, institution / company, research
group / department, address, phone, email)
- Desired virus type
(lentivirus or retrovirus)
- Gene name
- Accession number
(Refseq)
- Number of constructs
- Product volume (Mini, Midi,
Maxi, Concentrated)
- Construct name
- Vector DNA
concentration (µg/ml) (client provided vectors only)
- Biosafety
and virus titer analyses requested
Orders related to shRNA viral particles
To make an order for shRNA/shRNAmir containing viral particles, provide
BMGen a list of RefSeq numbers using virus construct information sheet and attach it to
the web form. For gene search tools, check NCBI, HGNC or MGI for the
latest RefSeq numbers associated with your target gene. Because all shRNA
constructs are targeted against mRNA transcripts, all RefSeq numbers
should start with “NM” or “XM” For example, RefSeq ID for BAK1 is
NM_001188.x
>> Delivery of the Products
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