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Biomedicum Genomics Oy
support@bmgen.fi
Haartmaninkatu 8
FI-00290 Helsinki
Finland
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Next-Generation Sequencing
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 BMGen offers next-generation sequencing services using
Illumina Genome Analyzer IIx (GAIIx) instrument by single-read option. The
read length is up to 100 base pairs. Next-generation sequencing can be
used in a wide variety of applications e.g:
- Whole genome sequencing and re-sequencing
- Targeted genome
sequencing
- Gene expression profiling (RNA-seq)
- Discovery
and analysis of small RNA (miRNA-seq)
- Chromatin
immunoprecipitation sequencing (ChIP-seq)
BMGen provides services
in all steps of next-generation sequencing by GAIIx:
- Sample preparation
- Target amplification / cluster
generation
- Sequencing
- Data analysis
In case the
customer wishes to run the data analysis by themselves, it is also
possible to retrieve the complete set of raw data files generated during
the experiment run. On request, BMGen also provides bioinformatics
assistance in downstream data analyses.
For more information on Genome Analyzer IIx sequencing and the related
services, please contact BMGen
support.
Targeted Sequence Capture
BMGen has now available the Roche Nimblegen’s array-based DNA capturing
system for targeted genome sequencing. Nimblegen arrays contain long
oligos (up to 60–mer) targeting e.g the whole human exome or customized
regions of the genome, where the target genome regions under interest can
be chosen. The array formats include:
- Human exome 2.1 M Array
- Customized arrays 2.1M or 385K
features per array
The sequence capture application provided by
BMGen allows to enrich specific regions of interest for next-generation
sequencing with high coverage and specificity, e.g. 2.1 M array captures
up to 30 Mb of genomic DNA.
Sample preparation and library construction can be ordered from BMGen.
Customer can also provide own samples that are compatible for single read
runs using GAIIx.
Please visit the Nimblegen website for detailed information on different
array formats.
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