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Biomedicum Genomics Oy
support@bmgen.fi
Haartmaninkatu 8
FI-00290 Helsinki
Finland
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Recombinant Virus Technology
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RNAi (shRNA/miRNA) and ORF (cDNA) expression constructs can be efficiently
introduced into most cell types by using recombinant viruses as vector
carriers. Recombinant virus transduction technology has a wide range of
applications in molecular and cell biology research and functional
genomics. Viruses are already used as routine gene construct delivery
tools in many laboratories, and there are many applications of viral
delivery systems such as:
- gene therapy research
- genetic or genomic level
screening
- discovery and validation of druggable
genes
- pathway identification
BMGen has acquired both
mouse and human TRC lentiviral
shRNA libraries encompassing 160 000 constructs for gene silencing
purposes.
Note! Recombinant virus particles are Biosafety Level 2 (BSL2) material,
and need to be maintained at BSL2 premises. Infected cells can be
transferred into normal laboratory conditions only after the replication
incompetence of the virus products in the target cells has been tested.
Transfer vectors used in production of recombinant viruses
Transfer vectors express shRNA, miRNA adapted shRNA or mRNA from DNA
inserts, which have been cloned into a virus vector backbone. These
constructs are designed to either silence or ectopically express the gene
of interest. When using BMGen, a customer can either supply us with
his/her own virus vectors or let BMGen provide them as a service.
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